A Chemical Toolbox for Labeling and Degrading Engineered Cas Proteins.

ABSTRACT: The discovery of clustered regularly interspaced short palindromic repeats and their associated proteins (Cas) has revolutionized the field of genome and epigenome editing. A number of new methods have been developed to precisely control the function and activity of Cas proteins, including fusion proteins and small-molecule modulators. Proteolysis-targeting chimeras (PROTACs) represent a new concept using the ubiquitin-proteasome system to degrade a protein of interest, highlighting the significance of chemically induced protein-E3 ligase interaction in drug discovery. Here, we engineered Cas proteins (Cas9, dCas9, Cas12, and Cas13) by inserting a Phe-Cys-Pro-Phe (FCPF) amino acid sequence (known as the π-clamp system) and demonstrate that the modified Cas^FCPF proteins can be (1

SUBMITTER: Gama-Brambila RA 

PROVIDER: S-EPMC8395650 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature