Intraoperative detection of isocitrate dehydrogenase mutations in human gliomas using a miniature mass spectrometer.

ABSTRACT: Knowledge of the isocitrate dehydrogenase (IDH) mutation status of glioma patients could provide insights for decision-making during brain surgery. However, pathology is not able to provide such information intraoperatively. Here we describe the first application of a miniature mass spectrometer (MS) to the determination of IDH mutation status in gliomas intraoperatively. The instrumentation was modified to be compatible with use in the operating room. Tandem MS was performed on the oncometabolite, 2-hydroxyglutarate, and a reference metabolite, glutamate, which is not involved in the IDH mutation. Ratios of fragment ion intensities were measured to calculate an IDH mutation score, which was used to differentiate IDH mutant and wild-type tissues. The results of analyzing 25 biopsies from 1

SUBMITTER: Brown HM 

PROVIDER: S-EPMC8396075 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature