Mechanisms of Viscous Media Effects on Elementary Steps of Bacterial Bioluminescent Reaction.

ABSTRACT: Enzymes activity in a cell is determined by many factors, among which viscosity of the microenvironment plays a significant role. Various cosolvents can imitate intracellular conditions in vitro, allowing to reduce a combination of different regulatory effects. The aim of the study was to analyze the media viscosity effects on the rate constants of the separate stages of the bacterial bioluminescent reaction. Non-steady-state reaction kinetics in glycerol and sucrose solutions was measured by stopped-flow technique and analyzed with a mathematical model developed in accordance with the sequence of reaction stages. Molecular dynamics methods were applied to reveal the effects of cosolvents on luciferase structure. We observed both in glycerol and in sucrose media that the stages of lucifera

SUBMITTER: Lisitsa AE 

PROVIDER: S-EPMC8396235 | biostudies-literature | 2021 Aug

REPOSITORIES: biostudies-literature