Applicability of Scrape Loading-Dye Transfer Assay for Non-Genotoxic Carcinogen Testing.

ABSTRACT: Dysregulation of gap junction intercellular communication (GJIC) is recognized as one of the key hallmarks for identifying non-genotoxic carcinogens (NGTxC). Currently, there is a demand for in vitro assays addressing the gap junction hallmark, which would have the potential to eventually become an integral part of an integrated approach to the testing and assessment (IATA) of NGTxC. The scrape loading-dye transfer (SL-DT) technique is a simple assay for the functional evaluation of GJIC in various in vitro cultured mammalian cells and represents an interesting candidate assay. Out of the various techniques for evaluating GJIC, the SL-DT assay has been used frequently to assess the effects of various chemicals on GJIC in toxicological and tumor promotion research. In this review, we system

SUBMITTER: Sovadinova I 

PROVIDER: S-EPMC8396440 | biostudies-literature | 2021 Aug

REPOSITORIES: biostudies-literature