Investigation of Bemethyl Biotransformation Pathways by Combination of LC-MS/HRMS and In Silico Methods.

ABSTRACT: Bemethyl is an actoprotector, an antihypoxant, and a moderate psychostimulant. Even though the therapeutic effectiveness of bemethyl is well documented, there is a gap in knowledge regarding its metabolic products and their quantitative and qualitative characteristics. Since 2018, bemethyl is included to the Monitoring Program of the World Anti-Doping Agency, which highlights the challenge of identifying its urinary metabolites. The objective of the study was to investigate the biotransformation pathways of bemethyl using a combination of liquid chromatography-high-resolution mass spectrometry and in silico studies. Metabolites were analyzed in a 24 h rat urine collected after oral administration of bemethyl at a single dose of 330 mg/kg. The urine samples were prepared for analysis by a p

SUBMITTER: Belinskaia DA 

PROVIDER: S-EPMC8396642 | biostudies-literature | 2021 Aug

REPOSITORIES: biostudies-literature