Single-cell RNA-sequencing reveals pre-meiotic X-chromosome dosage compensation in Drosophila testis.

ABSTRACT: Dosage compensation equalizes X-linked expression between XY males and XX females. In male fruit flies, expression levels of the X-chromosome are increased approximately two-fold to compensate for their single X chromosome. In testis, dosage compensation is thought to cease during meiosis; however, the timing and degree of the resulting transcriptional suppression is difficult to separate from global meiotic downregulation of each chromosome. To address this, we analyzed testis single-cell RNA-sequencing (scRNA-seq) data from two Drosophila melanogaster strains. We found evidence that the X chromosome is equally transcriptionally active as autosomes in somatic and pre-meiotic cells, and less transcriptionally active than autosomes in meiotic and post-meiotic cells. In cells experiencing do

SUBMITTER: Witt E 

PROVIDER: S-EPMC8396764 | biostudies-literature | 2021 Aug

REPOSITORIES: biostudies-literature