Paper-based ELISA diagnosis technology for human brucellosis based on a multiepitope fusion protein.

ABSTRACT:

Background

Brucellosis, as a serious zoonotic infectious disease, has been recognized as a re-emerging disease in the developing countries worldwide. In china, the incidence of brucellosis is increasing each year, seriously threatening the health of humans as well as animal populations. Despite a quite number of diagnostic methods currently being used for brucellosis, innovative technologies are still needed for its rapid and accurate diagnosis, especially in area where traditional diagnostic is unavailable.

Methodology/principal findings

In this study, a total of 22 B cell linear epitopes were predicted from five Brucella outer membrane proteins (OMPs) using an immunoinformatic approach. These epitopes were then chemically synthesized, and with the method of indirect ELISA

SUBMITTER: Yin D 

PROVIDER: S-EPMC8396774 | biostudies-literature | 2021 Aug

REPOSITORIES: biostudies-literature