SOX10 ablation severely impairs the generation of postmigratory neural crest from human pluripotent stem cells.

ABSTRACT: Animal studies have indicated that SOX10 is one of the key transcription factors regulating the proliferation, migration and differentiation of multipotent neural crest (NC), and mutation of SOX10 in humans may lead to type 4 Waardenburg syndrome (WS). However, the exact role of SOX10 in human NC development and the underlying molecular mechanisms of SOX10-related human diseases remain poorly understood due to the lack of appropriate human model systems. In this study, we successfully generated SOX10-knockout human induced pluripotent stem cells (SOX10^-/- hiPSCs) by the CRISPR-Cas9 gene editing tool. We found that loss of SOX10 significantly inhibited the generation of p75^highHNK1⁺/CD49D⁺ postmigratory neural crest stem cells (NCSCs) and upregula

SUBMITTER: Lai X 

PROVIDER: S-EPMC8397771 | biostudies-literature | 2021 Aug

REPOSITORIES: biostudies-literature