Effect of siRNA targeting dengue virus genes on replication of dengue virus: an in vitro experimental study.

ABSTRACT: Dengue is a notorious viral infection, which affects a large segment of world populations in absence of vaccines and anti-viral treatment. The current study evaluates role of effective siRNA in dengue virus replication. Eight siRNA were synthesized against five different genes (Capsid, CprM, NS1, NS3 and NS5) of all serotypes of dengue virus. All serotype of DV were transfected with all synthesized siRNA in vitro, using BHK-21 cell lines. Culture fluid from test and control was tested by Real time PCR for CT value comparison in siRNA treated cell line (test) and untreated cell line (controls). Percent knockdown (%KD) was calculated by ∆∆C_T methods to know the difference in test and control CT value. It was found that siRNA targeted against capsid gene worked best and showed inhi

SUBMITTER: Prakash O 

PROVIDER: S-EPMC8397848 | biostudies-literature | 2021 Sep

REPOSITORIES: biostudies-literature