Imaging carbonic anhydrase IX as a method for monitoring hypoxia-related radioresistance in preclinical head and neck cancer models.

ABSTRACT:

Background and purpose

Tumor hypoxia is an important cause of radioresistance and is associated with poor outcome.SPECT (Single-photon emission computed tomography) imaging enables visualizing tumor characteristics. We investigated the SPECT-radiotracer [¹¹¹In]-girentuximab-F(ab')₂ to image Carbonic Anhydrase IX (CAIX), an enzyme upregulated under hypoxic conditions.

Materials and methods

Athymic mice with subcutaneous FaDu or SCCNij202 head and neck squamous cell carcinoma (HNSCC) xenografts were treated with atovaquone or were housed in a hypoxic chamber (8% O₂). Next, [¹¹¹In]-girentuximab-F(ab')₂ was injected and 24 h later mice were euthanized for ex vivo biodistribution, autoradiography of the tumor, and immu

SUBMITTER: Huizing FJ 

PROVIDER: S-EPMC8397885 | biostudies-literature | 2021 Jul

REPOSITORIES: biostudies-literature