How to Turn an Electron Transfer Protein into a Redox Enzyme for Biosensing.

ABSTRACT: Cytochrome c is a small globular protein whose main physiological role is to shuttle electrons within the mitochondrial electron transport chain. This protein has been widely investigated, especially as a paradigmatic system for understanding the fundamental aspects of biological electron transfer and protein folding. Nevertheless, cytochrome c can also be endowed with a non-native catalytic activity and be immobilized on an electrode surface for the development of third generation biosensors. Here, an overview is offered of the most significant examples of such a functional transformation, carried out by either point mutation(s) or controlled unfolding. The latter can be induced chemically or upon protein immobilization on hydrophobic self-assembled monolayers. We critically discuss the p

SUBMITTER: Ranieri A 

PROVIDER: S-EPMC8398203 | biostudies-literature | 2021 Aug

REPOSITORIES: biostudies-literature