Protein Crystallization in a Microfluidic Contactor with Nafion^®117 Membranes.

ABSTRACT: Protein crystallization still remains mostly an empirical science, as the production of crystals with the required quality for X-ray analysis is dependent on the intensive screening of the best protein crystallization and crystal's derivatization conditions. Herein, this demanding step was addressed by the development of a high-throughput and low-budget microfluidic platform consisting of an ion exchange membrane (117 Nafion^® membrane) sandwiched between a channel layer (stripping phase compartment) and a wells layer (feed phase compartment) forming 75 independent micro-contactors. This microfluidic device allows for a simultaneous and independent screening of multiple protein crystallization and crystal derivatization conditions, using Hen Egg White Lysozyme (HEWL) as the model

SUBMITTER: Polino M 

PROVIDER: S-EPMC8398885 | biostudies-literature | 2021 Jul

REPOSITORIES: biostudies-literature