Tear Proteomics in Keratoconus: A Quantitative SWATH-MS Analysis.

ABSTRACT:

Purpose

To elucidate dysregulated proteins in keratoconus (KC) to provide a better understanding of the molecular mechanisms that lead to the development of the disease using sequential window acquisition of all theoretical mass spectra (SWATH-MS) as a protein quantification tool of the tear proteomic profile.

Methods

Prospective cross-sectional study that includes 25 keratoconic eyes and 25 healthy eyes. All participants underwent a clinical, tomographic, and aberrometric exam. Tear sample was collected using Schirmer strips and analyzed by liquid chromatography with tandem mass spectrometry. SWATH-MS was used as a quantification tool of the tear proteomic profile. The expression of the quantified proteins was compared between groups, and the biological and molecular functi

SUBMITTER: Lopez-Lopez M 

PROVIDER: S-EPMC8399462 | biostudies-literature | 2021 Aug

REPOSITORIES: biostudies-literature