Tea Bags for Fmoc Solid-Phase Peptide Synthesis: An Example of Circular Economy.

ABSTRACT: Peptide synthesis is an area with a wide field of application, from biomedicine to nanotechnology, that offers the option of simultaneously synthesizing a large number of sequences for the purpose of preliminary screening, which is a powerful tool. Nevertheless, standard protocols generate large volumes of solvent waste. Here, we present a protocol for the multiple Fmoc solid-phase peptide synthesis in tea bags, where reagent recycling steps are included. Fifty-two peptides with wide amino acid composition and seven to twenty amino acid residues in length were synthesized in less than three weeks. A clustering analysis was performed, grouping the peptides by physicochemical features. Although a relationship between the overall yield and the physicochemical features of the sequences was not

SUBMITTER: Guzman F 

PROVIDER: S-EPMC8399505 | biostudies-literature | 2021 Aug

REPOSITORIES: biostudies-literature