The role of the electrokinetic charge of neurotrophis-based nanocarriers: protein distribution, toxicity, and oxidative stress in in vitro setting.

ABSTRACT:

Background

The rational chemical design of nanoparticles can be readily controlled and optimized by quantitatively studying protein adsorption at variously charged polymer carriers, determining their fate in biological fluids. We manufactured brain-derived neurotrophic factor (BDNF) -based electrostatic nanocomplexes with a different type of dendrimer core (anionic or cationic), encapsulated or not in polyethylene glycol (PEG), and studied their physicochemical properties and behavior in a biological setting. We investigated whether the electrokinetic charge of dendrimer core influences BDNF loading and desorption from the nanoparticle and serves as a determinant of nanoparticles' behavior in in vitro setting, influencing mitochondrial dysfunction, lipid peroxidation, and general n

SUBMITTER: Dabkowska M 

PROVIDER: S-EPMC8399784 | biostudies-literature | 2021 Aug

REPOSITORIES: biostudies-literature