Metabolomic Evaluation of Tissue-Specific Defense Responses in Tomato Plants Modulated by PGPR-Priming against Phytophthora capsici Infection.

ABSTRACT: Plant growth-promoting rhizobacteria (PGPR) can stimulate disease suppression through the induction of an enhanced state of defense readiness. Here, untargeted ultra-high performance liquid chromatography-mass spectrometry (UHPLC-MS) and targeted ultra-high performance liquid chromatography coupled to triple-quadrupole mass spectrometry (UHPLC-QqQ-MS) were used to investigate metabolic reprogramming in tomato plant tissues in response to priming by Pseudomonas fluorescens N04 and Paenibacillus alvei T22 against Phytophthora capsici. Roots were treated with the two PGPR strains prior to stem inoculation with Ph. capsici. Metabolites were methanol-extracted from roots, stems and leaves at two-eight days post-inoculation. Targeted analysis by UHPLC-QqQ-MS allowed quantification of aromatic am

SUBMITTER: Mhlongo MI 

PROVIDER: S-EPMC8400099 | biostudies-literature | 2021 Jul

REPOSITORIES: biostudies-literature