Consideration of Metabolite Efflux in Radiolabelled Choline Kinetics.

ABSTRACT: Hypoxia is a complex microenvironmental condition known to regulate choline kinase α (CHKA) activity and choline transport through transcription factor hypoxia-inducible factor-1α (HIF-1α) and, therefore, may confound the uptake of choline radiotracer [¹⁸F]fluoromethyl-[1,2-²H₄]-choline ([¹⁸F]-D4-FCH). The aim of this study was to investigate how hypoxia affects the choline radiotracer dynamics. Three underlying mechanisms by which hypoxia could potentially alter the uptake of the choline radiotracer, [¹⁸F]-D4-FCH, were investigated: ¹⁸F-D4-FCH import, CHKA phosphorylation activity, and the efflux of [¹⁸F]-D4-FCH and its phosphorylated product [¹⁸F]-D4-FCHP. The effects of hypoxia on [¹⁸F]-D4

SUBMITTER: Li Y 

PROVIDER: S-EPMC8400349 | biostudies-literature | 2021 Aug

REPOSITORIES: biostudies-literature