Modelling and Differential Quantification of Electric Cell-Substrate Impedance Sensing Growth Curves.

ABSTRACT: Measurement of cell surface coverage has become a common technique for the assessment of growth behavior of cells. As an indirect measurement method, this can be accomplished by monitoring changes in electrode impedance, which constitutes the basis of electric cell-substrate impedance sensing (ECIS). ECIS typically yields growth curves where impedance is plotted against time, and changes in single cell growth behavior or cell proliferation can be displayed without significantly impacting cell physiology. To provide better comparability of ECIS curves in different experimental settings, we developed a large toolset of R scripts for their transformation and quantification. They allow importing growth curves generated by ECIS systems, edit, transform, graph and analyze them while delivering q

SUBMITTER: Binder ARD 

PROVIDER: S-EPMC8401457 | biostudies-literature | 2021 Aug

REPOSITORIES: biostudies-literature