Microscopic Object Classification through Passive Motion Observations with Holographic Microscopy.

ABSTRACT: Digital holographic microscopy provides the ability to observe throughout a volume that is large compared to its resolution without the need to actively refocus to capture the entire volume. This enables simultaneous observations of large numbers of small objects within such a volume. We have constructed a microscope that can observe a volume of 0.4 µm × 0.4 µm × 1.0 µm with submicrometer resolution (in xy) and 2 µm resolution (in z) for observation of microorganisms and minerals in liquid environments on Earth and on potential planetary missions. Because environmental samples are likely to contain mixtures of inorganics and microorganisms of comparable sizes near the resolution limit of the instrument, discrimination between living and non-living objects may be difficult. The active motio

SUBMITTER: Rouzie D 

PROVIDER: S-EPMC8401815 | biostudies-literature | 2021 Aug

REPOSITORIES: biostudies-literature