Clinical Mass Spectrometry Discovered Human IgG Sialylation as a Potential Biosignature for Kidney Function.

ABSTRACT: Immunoglobulin G (IgG) N-glycosylation was discovered to have an association with inflammation status, which has the potential to be a novel biomarker for kidney diseases. In this study, we applied an ultra-high performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) method to plasma and urine samples from 57 individuals with different levels of kidney function. Natural abundances of total IgG, IgG1, IgG2, and IgG3 subclasses in plasma showed positive correlations to the estimated glomerular filtration rates (eGFRs). Eighteen IgG glycopeptides also showed positive correlations. In contrast, higher IgG amounts were found in urine samples from participants with lower eGFR values. After normalizing IgG glycopeptides from plasma to their respective protein amounts, H4N4F1S1-Ig

SUBMITTER: Kao CC 

PROVIDER: S-EPMC8401842 | biostudies-literature | 2021 Jul

REPOSITORIES: biostudies-literature