A Genetically Encoded Isonitrile Lysine for Orthogonal Bioorthogonal Labeling Schemes.

ABSTRACT: Bioorthogonal click-reactions represent ideal means for labeling biomolecules selectively and specifically with suitable small synthetic dyes. Genetic code expansion (GCE) technology enables efficient site-selective installation of bioorthogonal handles onto proteins of interest (POIs). Incorporation of bioorthogonalized non-canonical amino acids is a minimally perturbing means of enabling the study of proteins in their native environment. The growing demand for the multiple modification of POIs has triggered the quest for developing orthogonal bioorthogonal reactions that allow simultaneous modification of biomolecules. The recently reported bioorthogonal [4 + 1] cycloaddition reaction of bulky tetrazines and sterically demanding isonitriles has prompted us to develop a non-canonical amin

SUBMITTER: Szatmari A 

PROVIDER: S-EPMC8402055 | biostudies-literature | 2021 Aug

REPOSITORIES: biostudies-literature