Changes of Viscoelastic Properties of Aptamer-Based Sensing Layers Following Interaction with Listeria innocua.

ABSTRACT: A multiharmonic quartz crystal microbalance (QCM) has been applied to study the viscoelastic properties of the aptamer-based sensing layers at the surface of a QCM transducer covered by neutravidin following interaction with bacteria Listeria innocua. Addition of bacteria in the concentration range 5 × 10³-10⁶ CFU/mL resulted in a decrease of resonant frequency and in an increase of dissipation. The frequency decrease has been lower than one would expect considering the dimension of the bacteria. This can be caused by lower penetration depth of the acoustics wave (approximately 120 nm) in comparison with the thickness of the bacterial layer (approximately 500 nm). Addition of E. coli at the surface of neutravidin as well as aptamer layers did not result

SUBMITTER: Tatarko M 

PROVIDER: S-EPMC8402281 | biostudies-literature | 2021 Aug

REPOSITORIES: biostudies-literature