Inferring Numbers of Wild Poliovirus Excretors Using Quantitative Environmental Surveillance.

ABSTRACT: Response to and monitoring of viral outbreaks can be efficiently focused when rapid, quantitative, kinetic information provides the location and the number of infected individuals. Environmental surveillance traditionally provides information on location of populations with contagious, infected individuals since infectious poliovirus is excreted whether infections are asymptomatic or symptomatic. Here, we describe development of rapid (1 week turnaround time, TAT), quantitative RT-PCR of poliovirus RNA extracted directly from concentrated environmental surveillance samples to infer the number of infected individuals excreting poliovirus. The quantitation method was validated using data from vaccination with bivalent oral polio vaccine (bOPV). The method was then applied to infer the weekly

SUBMITTER: Perepliotchikov Y 

PROVIDER: S-EPMC8402366 | biostudies-literature | 2021 Aug

REPOSITORIES: biostudies-literature