Bioprinted Multi-Cell Type Lung Model for the Study of Viral Inhibitors.

ABSTRACT: Influenza A virus (IAV) continuously causes epidemics and claims numerous lives every year. The available treatment options are insufficient and the limited pertinence of animal models for human IAV infections is hampering the development of new therapeutics. Bioprinted tissue models support studying pathogenic mechanisms and pathogen-host interactions in a human micro tissue environment. Here, we describe a human lung model, which consisted of a bioprinted base of primary human lung fibroblasts together with monocytic THP-1 cells, on top of which alveolar epithelial A549 cells were printed. Cells were embedded in a hydrogel consisting of alginate, gelatin and collagen. These constructs were kept in long-term culture for 35 days and their viability, expression of specific cell markers and

SUBMITTER: Berg J 

PROVIDER: S-EPMC8402746 | biostudies-literature | 2021 Aug

REPOSITORIES: biostudies-literature