Comparison and Evaluation of Real-Time Taqman PCR for Detection and Quantification of Ebolavirus.

ABSTRACT: Given that ebolavirus causes severe and frequently lethal disease, its rapid and accurate detection using available and validated methods is essential for controlling infection. Real-time reverse-transcription PCR (RT-PCR) has proven to be an invaluable tool for ebolaviruses diagnostics. Many assays with different targets have been developed, but they have not been externally compared or validated, and limits of detection are not uniformly reported. Here we compared and evaluated the sensitivity, reproducibility and specificity of 23 in-house assays under the same conditions. Our results showed that these assays were highly gene- and species- specific when evaluated using in vitro RNA transcripts and viral RNA, and the potential limits of detection were uniformly reported ranging from 10

SUBMITTER: Huang Y 

PROVIDER: S-EPMC8402893 | biostudies-literature | 2021 Aug

REPOSITORIES: biostudies-literature