Immunoresponsive Tissue-Engineered Oral Mucosal Equivalents Containing Macrophages.

ABSTRACT: Macrophages play a key role in orchestrating the host immune response toward invading organisms or non-self molecules in the oral mucosa. Three-dimensional (3D) oral mucosal equivalents (OME) containing oral fibroblasts and keratinocytes are used extensively to mimic the human oral mucosa where they have been employed to examine innate immune responses to both bacterial and fungal pathogens as well as to biomaterials. Although the presence of immune cells is critical in generating an immune response, very few studies have incorporated leukocytes into OME, and to date, none have contained primary human macrophages. In this study, we report the generation of an immunocompetent OME to investigate immune responses toward bacterial challenge. Primary human monocyte-derived macrophages (MDM) wer

SUBMITTER: Ollington B 

PROVIDER: S-EPMC8403184 | biostudies-literature | 2021 Aug

REPOSITORIES: biostudies-literature