Induction of Ventral Spinal V0 Interneurons from Mouse Embryonic Stem Cells.

ABSTRACT: The ventral spinal population of V0 interneurons (INs) contributes to the coordinated movements directed by spinal central pattern generators (CPGs), including respiratory circuits and left-right alternation in locomotion. One challenge in studying V0 INs has been the limited number of cells that can be isolated from primary sources for basic research or therapeutic use. However, derivation from a pluripotent source, such as has been done recently for other IN populations, could resolve this issue. However, there is currently no protocol to specifically derive V0 interneurons from pluripotent cell types. To generate an induction protocol, mouse embryonic stem cells (mESCs) were grown in suspension culture and then exposed to retinoic acid (RA) and collected at different time points to meas

SUBMITTER: Pardieck J 

PROVIDER: S-EPMC8403187 | biostudies-literature | 2021 Aug

REPOSITORIES: biostudies-literature