Combining protein and metabolic engineering strategies for biosynthesis of melatonin in Escherichia coli.

ABSTRACT:

Background

Melatonin has attracted substantial attention because of its excellent prospects for both medical applications and crop improvement. The microbial production of melatonin is a safer and more promising alternative to chemical synthesis approaches. Researchers have failed to produce high yields of melatonin in common heterologous hosts due to either the insolubility or low enzyme activity of proteins encoded by gene clusters related to melatonin biosynthesis.

Results

Here, a combinatorial gene pathway for melatonin production was successfully established in Escherichia coli by combining the physostigmine biosynthetic genes from Streptomyces albulus and gene encoding phenylalanine 4-hydroxylase (P4H) from Xanthomonas campestris and caffeic acid 3-O-methyltransferase

SUBMITTER: Zhang Y 

PROVIDER: S-EPMC8403405 | biostudies-literature | 2021 Aug

REPOSITORIES: biostudies-literature