Multiple acid-base and electrolyte disturbances upregulate NBCn1, NBCn2, IRBIT and L-IRBIT in the mTAL.

ABSTRACT:

Key points

The roles of the Na⁺ /HCO₃^- cotransporters NBCn1 and NBCn2 as well as their activators IRBIT and L-IRBIT in the regulation of the mTAL transport of NH₄⁺ , HCO₃^- , and NaCl are investigated. Dietary challenges of NH₄ Cl, NaHCO₃ or NaCl all increase the abundance of NBCn1 and NBCn2 in the outer medulla. The three challenges generally produce parallel increases in the abundance of IRBIT and L-IRBIT in the outer medulla. Both IRBIT and L-IRBIT powerfully stimulate the activities of the mTAL isoforms of NBCn1 and NBCn2 as expressed in Xenopus oocytes. Our findings support the hypothesis that NBCn1, NBCn2, IRBIT and L-IRBIT appropriately promote NH₄⁺ shuntin

SUBMITTER: Wang JL 

PROVIDER: S-EPMC8403746 | biostudies-literature | 2020 Aug

REPOSITORIES: biostudies-literature