Assisted assembly of bacteriophage T7 core components for genome translocation across the bacterial envelope.

ABSTRACT: In most bacteriophages, genome transport across bacterial envelopes is carried out by the tail machinery. In viruses of the Podoviridae family, in which the tail is not long enough to traverse the bacterial wall, it has been postulated that viral core proteins assembled inside the viral head are translocated and reassembled into a tube within the periplasm that extends the tail channel. Bacteriophage T7 infects Escherichia coli, and despite extensive studies, the precise mechanism by which its genome is translocated remains unknown. Using cryo-electron microscopy, we have resolved the structure of two different assemblies of the T7 DNA translocation complex composed of the core proteins gp15 and gp16. Gp15 alone forms a partially folded hexamer, which is further assembled upo

SUBMITTER: Perez-Ruiz M 

PROVIDER: S-EPMC8403907 | biostudies-literature | 2021 Aug

REPOSITORIES: biostudies-literature