The Stability Landscape of de novo TIM Barrels Explored by a Modular Design Approach.

ABSTRACT: The ability to design stable proteins with custom-made functions is a major goal in biochemistry with practical relevance for our environment and society. Understanding and manipulating protein stability provide crucial information on the molecular determinants that modulate structure and stability, and expand the applications of de novo proteins. Since the (β/⍺)₈-barrel or TIM-barrel fold is one of the most common functional scaffolds, in this work we designed a collection of stable de novo TIM barrels (DeNovoTIMs), using a computational fixed-backbone and modular approach based on improved hydrophobic packing of sTIM11, the first validated de novo TIM barrel, and subjected them to a thorough folding analysis. DeNovoTIMs navigate a region of the stability landscape previously u

SUBMITTER: Romero-Romero S 

PROVIDER: S-EPMC8404036 | biostudies-literature | 2021 Sep

REPOSITORIES: biostudies-literature