Detection of African swine fever virus in pigs in Southwest Nigeria.

ABSTRACT:

Background and aim

Nigeria experienced repeated outbreaks of African swine fever (ASF) in pig herds between 1997 and 2005 in the southwest region of the country. ASF is believed to currently be enzootic in this region. The status of enzootic transmission of ASF virus strain to pigs is; however, unknown. Twenty-three genotypes of the ASF virus based on the p72 gene are found across Africa. This study aimed to identify the current circulating field strain(s) of the ASF virus in Southwest Nigeria and characterized evolutionary trends.

Materials and methods

DNA samples were extracted from 144 pooled blood samples obtained from 2012 to 2013 following the manufacturer's instructions. DNA was used for conventional polymerase chain reaction using primers targeting the p72<

SUBMITTER: Awosanya EJ 

PROVIDER: S-EPMC8404123 | biostudies-literature | 2021 Jul

REPOSITORIES: biostudies-literature