Cancer-associated Fibroblast-derived Spondin-2 Promotes Motility of Gastric Cancer Cells.

ABSTRACT:

Background/aim

Peritoneal dissemination (PD) occurs frequently in gastric cancer (GC) and is fatal. The interactions between tumor cells and stromal cells are critical for cancer progression. Our aim was to identify a novel PD-associated gene derived from stromal cells in GC.

Materials and methods

Among the candidate PD-associated genes identified in our previous study, we focused on spondin-2 (SPON2), an extracellular matrix-secreted protein. Clinicopathological and prognostic analyses of SPON2 mRNA expression were performed using GC datasets. Localization of SPON2 expression was assessed by immunohistochemistry. In vitro migration assay and immunofluorescence staining were also conducted using GC cell lines.

Results

SPON2 was expressed in and secreted from cancer-as

SUBMITTER: Kuramitsu S 

PROVIDER: S-EPMC8404736 | biostudies-literature | 2021 Jul-Aug

REPOSITORIES: biostudies-literature