Molecular characterization of Blastocystis subtypes isolated in the city of Uberaba, Minas Gerais State, Brazil.

ABSTRACT:

Introduction

Blastocystis is an intestinal protozoan that may play a role in the pathogenicity of humans. This study aimed to (i) genetically characterize Blastocystis isolates obtained from human fecal samples and the water supply of the city of Uberaba, Minas Gerais, Brazil, and (ii) to verify the phylogenetic relationship between these isolates.

Methods

Blastocystis species present in 26 fecal samples obtained from humans and animals from Uberaba were genetically characterized by polymerase chain reaction-restriction fragment length polymorphism and polymerase chain reaction-sequence-tagged sites. All amplicons were partially sequenced and/or defined according to the GenBank classification.

Results

Polymerase chain reaction amplicons were generated from 21 human is

SUBMITTER: Cabrine-Santos M 

PROVIDER: S-EPMC8405216 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature