Detection of Borrelia burgdorferi antigens in tissues and plasma during early infection in a mouse model.

ABSTRACT: Borrelia burgdorferi is the causative agent of Lyme borreliosis, which is the most common tick-borne human disease in Europe and North America. Currently, the diagnosis of Lyme borreliosis is based on serological tests allowing indirect detection of anti-Borrelia antibodies produced by patients. Their main drawback is a lack of sensitivity in the early phase of disease and an incapacity to prove an active infection. Direct diagnostic tests are clearly needed. The objectives of this study were to produce tools allowing sensitive detection of potential circulating Borrelia antigens and to evaluate them in a mouse model. We focused on two potential early bacterial makers, the highly variable OspC protein and the conserved protein FlaB. High-affinity monoclonal antibodies were produced and use

SUBMITTER: Dolange V 

PROVIDER: S-EPMC8405660 | biostudies-literature | 2021 Aug

REPOSITORIES: biostudies-literature