Protocol for targeting the magnocellular neuroendocrine cell ensemble via retrograde tracing from the posterior pituitary.
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ABSTRACT:
SUBMITTER: Zhang B
PROVIDER: S-EPMC8405943 | biostudies-literature | 2021 Sep
REPOSITORIES: biostudies-literature
Publications
Protocol for targeting the magnocellular neuroendocrine cell ensemble via retrograde tracing from the posterior pituitary.
Zhang Bin B Qiu Liyao L Long Chaoying C Gao Zhihua Z
STAR protocols 20210827 3
The hypothalamic magnocellular neuroendocrine cells (MNCs) project to the posterior pituitary (PPi), regulating reproduction and fluid homeostasis. It has been challenging to selectively label and manipulate MNCs, as they are intermingled with parvocellular neuroendocrine cells projecting to the median eminence. Here, we provide a step-by-step protocol for specifically targeting the MNCs by infusing retrograde viral tracers into the PPi. When combined with optogenetics, chemogenetics, and transg ...[more]
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