Cysteine Mutants of the Major Facilitator Superfamily-Type Transporter CcoA Provide Insight into Copper Import.

ABSTRACT: CcoA belongs to the widely distributed bacterial copper (Cu) importer subfamily CalT (CcoA-like Transporters) of the Major Facilitator Superfamily (MFS) and provides cytoplasmic Cu needed for cbb₃-type cytochrome c oxidase (cbb₃-Cox) biogenesis. Earlier studies have supported a 12-transmembrane helix (TMH) topology of CcoA with the well-conserved Met₂₃₃xxxMet₂₃₇ and His₂₆₁xxxMet₂₆₅ motifs in its TMH7 and TMH8, respectively. Of these residues, Met₂₃₃ and His₂₆₁ are essential for Cu uptake and cbb₃-Cox production, whereas Met₂₃₇ and Met₂₆₅ contribute partly to these processes. CcoA also contains five Cys residues of u

SUBMITTER: Khalfaoui-Hassani B 

PROVIDER: S-EPMC8406296 | biostudies-literature | 2021 Aug

REPOSITORIES: biostudies-literature