Characterization of histone inheritance patterns in the Drosophila female germline.

ABSTRACT: Stem cells have the unique ability to undergo asymmetric division which produces two daughter cells that are genetically identical, but commit to different cell fates. The loss of this balanced asymmetric outcome can lead to many diseases, including cancer and tissue dystrophy. Understanding this tightly regulated process is crucial in developing methods to treat these abnormalities. Here, we report that during a Drosophila female germline stem cell asymmetric division, the two daughter cells differentially inherit histones at key genes related to either maintaining the stem cell state or promoting differentiation, but not at constitutively active or silenced genes. We combine histone labeling with DNA Oligopaints to distinguish old versus new histones and visualize their inheritance patte

SUBMITTER: Kahney EW 

PROVIDER: S-EPMC8406404 | biostudies-literature | 2021 Jul

REPOSITORIES: biostudies-literature