SHOX2 cooperates with STAT3 to promote breast cancer metastasis through the transcriptional activation of WASF3.

ABSTRACT:

Background

Metastasis is most often the root cause of cancer-related death. Human short stature homeobox 2 (SHOX2), a homeodomain transcription factor, is a novel inducer of epithelial-to-mesenchymal transition in breast cancer cells, though its exact role and underlying mechanisms in metastasis are not well understood.

Methods

TCGA analysis was performed to identify the clinical relevance of SHOX2 in breast cancer. Gene depletion was achieved by short hairpin RNA and small interfering RNA. Molecular regulations and alterations were assessed by Western blotting, immunoprecipitation, immunohistochemistry, qRT-PCR, chromatin immunoprecipitation coupled with qPCR (ChIP-qPCR), and ChIP/re-ChIP. The impact of SHOX2 signaling on tumor growth and metastasis was evaluated in orthoto

SUBMITTER: Teng Y 

PROVIDER: S-EPMC8406721 | biostudies-literature | 2021 Aug

REPOSITORIES: biostudies-literature