GFP-Tagged Protein Detection by Electron Microscopy Using a GBP-APEX Tool in Drosophila.

ABSTRACT: In cell biology, detection of protein subcellular localizations is often achieved by optical microscopy techniques and more rarely by electron microscopy (EM) despite the greater resolution offered by EM. One of the possible reasons was that protein detection by EM required specific antibodies whereas this need could be circumvented by using fluorescently-tagged proteins in optical microscopy approaches. Recently, the description of a genetically encodable EM tag, the engineered ascorbate peroxidase (APEX), whose activity can be monitored by electron-dense DAB precipitates, has widened the possibilities of specific protein detection in EM. However, this technique still requires the generation of new molecular constructions. Thus, we decided to develop a versatile method that would take adv

SUBMITTER: Bernard F 

PROVIDER: S-EPMC8406855 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature