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Phase-gradient microscopy in thick tissue with oblique back-illumination.

ABSTRACT: Phase-contrast techniques, such as differential interference contrast microscopy, are widely used to obtain morphological images of unstained biological samples. The transillumination geometry required for these techniques restricts their application to thin samples. We introduce oblique back-illumination microscopy, a method of collecting en face phase-gradient images of thick scattering samples, enabling near-video-rate in vivo phase imaging with a miniaturized probe suitable for endoscopy.

SUBMITTER: Ford TN

PROVIDER: S-EPMC8407656 | biostudies-literature | 2012 Dec

REPOSITORIES: biostudies-literature

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Phase-gradient microscopy in thick tissue with oblique back-illumination.

Ford Tim N TN Chu Kengyeh K KK Mertz Jerome J

Nature methods 20121028 12

Phase-contrast techniques, such as differential interference contrast microscopy, are widely used to obtain morphological images of unstained biological samples. The transillumination geometry required for these techniques restricts their application to thin samples. We introduce oblique back-illumination microscopy, a method of collecting en face phase-gradient images of thick scattering samples, enabling near-video-rate in vivo phase imaging with a miniaturized probe suitable for endoscopy. ...[more]

PMID: 23103879

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