Immunological detection of AcAMP antimicrobial peptide secreted by Aspergillus clavatus.

ABSTRACT:

Background and objectives

Aspergillus clavatus antimicrobial peptide (AcAMP) is a fungi-derived peptide with a broad spectrum of activity against pathogenic bacteria and fungi. Natural antimicrobial peptides, including AcAMP, have attracted many attentions in the development of new natural antibiotics against pathogenic bacteria, especially multidrug resistant ones.

Materials and methods

In the present study, acamp gene was codon-optimized and chemically synthesized in pUC57 cloning vector, subcloned into pET28a (+) expression vector and transferred into competent Escherichia coli BL21 (DE3) cells. The expression of AcAMP was induced by addition of Isopropyl β- d-1-thiogalactopyranoside (IPTG) and the expressed peptide was purified by Ni-NTA. BALB/c mice

SUBMITTER: Zamani E 

PROVIDER: S-EPMC8408025 | biostudies-literature | 2021 Apr

REPOSITORIES: biostudies-literature