FTO-mediated demethylation of GADD45B promotes myogenesis through the activation of p38 MAPK pathway.

ABSTRACT: N6-methyladenosine (m⁶A) modification plays a critical role in mammalian development. However, the role of m⁶A in the skeletal muscle development remains largely unknown. Here, we report a global m⁶A modification pattern of goat skeletal muscle at two key development stages and identified that the m⁶A modification regulated the expression of the growth arrest and DNA damage-inducible 45B (GADD45B) gene, which is involved in myogenic differentiation. We showed that GADD45B expression increased during myoblast differentiation, whereas the downregulation of GADD45B inhibits myogenic differentiation and mitochondrial biogenesis. Moreover, the expression of GADD45B regulates the expression of myogenic regulatory factors and peroxisome prolifera

SUBMITTER: Deng K 

PROVIDER: S-EPMC8408560 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature