Hippocampal glucose uptake as a surrogate of metabolic change of microglia in Alzheimer's disease.

ABSTRACT:

Background

Dynamically altered microglia play an important role in the progression of Alzheimer's disease (AD). Here, we found a close association of the metabolic reconfiguration of microglia with increased hippocampal glucose uptake on [¹⁸F]fluorodeoxyglucose (FDG) PET.

Methods

We used an AD animal model, 5xFAD, to analyze hippocampal glucose metabolism using both animal FDG PET and ex vivo FDG uptake test. Cells of the hippocampus were isolated to perform single-cell RNA-sequencing (scRNA-seq). The molecular features of cells associated with glucose metabolism were analyzed at a single-cell level. In order to apply our findings to human brain imaging study, brain FDG PET data obtained from the Alzheimer's Disease Neuroimaging Initiative were analyzed. FDG uptak

SUBMITTER: Choi H 

PROVIDER: S-EPMC8408933 | biostudies-literature | 2021 Aug

REPOSITORIES: biostudies-literature