Single-cell analysis of cell fate bifurcation in the chordate Ciona.

ABSTRACT:

Background

Inductive signaling interactions between different cell types are a major mechanism for the further diversification of embryonic cell fates. Most blastomeres in the model chordate Ciona robusta become restricted to a single predominant fate between the 64-cell and mid-gastrula stages. The deeply stereotyped and well-characterized Ciona embryonic cell lineages allow the transcriptomic analysis of newly established cell types very early in their divergence from sibling cell states without the pseudotime inference needed in the analysis of less synchronized cell populations. This is the first ascidian study to use droplet scRNAseq with large numbers of analyzed cells as early as the 64-cell stage when major lineages such as primary notochord first become fate restricted.

SUBMITTER: Winkley KM 

PROVIDER: S-EPMC8408944 | biostudies-literature | 2021 Aug

REPOSITORIES: biostudies-literature