Bioactivity screening of environmental chemicals using imaging-based high-throughput phenotypic profiling.

ABSTRACT: The present study adapted an existing high content imaging-based high-throughput phenotypic profiling (HTPP) assay known as "Cell Painting" for bioactivity screening of environmental chemicals. This assay uses a combination of fluorescent probes to label a variety of organelles and measures a large number of phenotypic features at the single cell level in order to detect chemical-induced changes in cell morphology. First, a small set of candidate phenotypic reference chemicals (n = 14) known to produce changes in the cellular morphology of U-2 OS cells were identified and screened at multiple time points in concentration-response format. Many of these chemicals produced distinct cellular phenotypes that were qualitatively similar to those previously described in the literature. A novel wor

SUBMITTER: Nyffeler J 

PROVIDER: S-EPMC8409064 | biostudies-literature | 2020 Jan

REPOSITORIES: biostudies-literature