Ca²⁺ -activated K⁺ channel K_Ca 1.1 as a therapeutic target to overcome chemoresistance in three-dimensional sarcoma spheroid models.

ABSTRACT: The large-conductance Ca²⁺ -activated K⁺ channel K_Ca 1.1 plays a pivotal role in tumor development and progression in several solid cancers. The three-dimensional (3D) in vitro cell culture system is a powerful tool for cancer spheroid formation, and mimics in vivo solid tumor resistance to chemotherapy in the tumor microenvironment (TME). K_Ca 1.1 is functionally expressed in osteosarcoma and chondrosarcoma cell lines. K_Ca 1.1 activator-induced hyperpolarizing responses were significantly larger in human osteosarcoma MG-63 cells isolated from 3D spheroid models compared with in those from adherent 2D monolayer cells. The present study investigated the mechanisms underlying the upregulation of K_Ca 1.1 and its role in chemores

SUBMITTER: Ohya S 

PROVIDER: S-EPMC8409426 | biostudies-literature | 2021 Sep

REPOSITORIES: biostudies-literature