High-Throughput Screening of Mouse Gene Knockouts Identifies Established and Novel High Body Fat Phenotypes.

ABSTRACT:

Purpose

Obesity is a major public health problem. Understanding which genes contribute to obesity may better predict individual risk and allow development of new therapies. Because obesity of a mouse gene knockout (KO) line predicts an association of the orthologous human gene with obesity, we reviewed data from the Lexicon Genome5000^TM high throughput phenotypic screen (HTS) of mouse gene KOs to identify KO lines with high body fat.

Materials and methods

KO lines were generated using homologous recombination or gene trapping technologies. HTS body composition analyses were performed on adult wild-type and homozygous KO littermate mice from 3758 druggable mouse genes having a human ortholog. Body composition was measured by either DXA or QMR on chow-fed cohorts fr

SUBMITTER: Powell DR 

PROVIDER: S-EPMC8409770 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature