Sensitive, homogeneous, and label-free protein-probe assay for antibody aggregation and thermal stability studies.

ABSTRACT: Protein aggregation is a spontaneous process affected by multiple external and internal properties, such as buffer composition and storage temperature. Aggregation of protein-based drugs can endanger patient safety due, for example, to increased immunogenicity. Aggregation can also inactivate protein drugs and prevent target engagement, and thus regulatory requirements are strict regarding drug stability monitoring during manufacturing and storage. Many of the current technologies for aggregation monitoring are time- and material-consuming and require specific instruments and expertise. These types of assays are not only expensive, but also unsuitable for larger sample panels. Here we report a label-free time-resolved luminescence-based method using an external Eu³⁺-conjugated p

SUBMITTER: Valtonen S 

PROVIDER: S-EPMC8409793 | biostudies-literature | 2021 Jan-Dec

REPOSITORIES: biostudies-literature