Diurnal properties of tonic and synaptic GABA_A receptor-mediated currents in suprachiasmatic nucleus neurons.

ABSTRACT: Synaptic and extrasynaptic GABA_A receptor (GABA_AR)-mediated neurotransmission is a critical component of the suprachiasmatic nucleus (SCN) neuronal network. However, the properties of the GABA_A tonic current (I_tonic) and its origin remain unexplored. Spontaneous GABA_A postsynaptic currents (sGPSCs) and I_tonic were recorded from SCN neurons with the whole cell voltage-clamp technique at different times of the day. GABA_AR antagonists (bicuculline, gabazine, and picrotoxin) inhibited sGPSC and induced an outward shift of the holding current, which defined the I_tonic amplitude. The sGPSC frequency, synaptic charge transfer, and I_tonic amplitude all demonstrated signifi

SUBMITTER: Moldavan M 

PROVIDER: S-EPMC8409954 | biostudies-literature | 2021 Aug

REPOSITORIES: biostudies-literature